The Lions are now the second Detroit sports franchise that have had to disavow the use of their logo at the Unite the Right rally in Charlottesville, Virginia.
A photo by Getty Images photographer Chip Somodevilla shows a man at the demonstration in Charlottesville's Emancipation Park beating a shield that bears a logo similar to the Lions' logo, although this one was blue and red and had stars on it.
"We detest and disavow any use or implies use of the Detroit Lions logo or any of our marks in association with the event this past Saturday in Charlottesville," the team said in a statement released Tuesday night.
"We value diversity as it represents the strong fabric of our team, the City of Detroit, the NFL, the game of football, our fans and our country."
The Detroit Red Wings issued a similar statement Saturday after their logo was used during the demonstration and said they are considering legal action.
The rally descended into violence after white nationalists, neo-Nazis and other far-right extremists traveled to the city to protest the removal of a statue of Confederate Gen. Robert E. Lee.
Amid the fighting between the demonstrators and counter protesters, Heather Heyer, a 32-year-old legal assistant from Charlottesville, was killed when a car plowed into a crowd of people protesting the white supremacist rally.
Police have charged 20-year-old James Alex Fields Jr. with second-degree murder for allegedly driving the car.