The Rays lost the game, 5-4 in 10 innings. But Tampa Bay manager Joe Maddon protested the game because of a fourth inning decision by umpiring crew chief Bob Davidson.
Davidson allowed Toronto manager John Gibbons to challenge a call at first base on an attempted picked off of Wil Myers. The call was overturned and Myers was call out.
Blue Jays pitcher Mark Buehrle was standing on the rubber and Yunel Escobar was standing in the batter's box before Gibbons left the dugout to challenge the safe call.
Maddon argued that because the players were in place, the challenge should not have been allowed.
"It's a legitimate protest," Maddon said. "Hitter in box, pitcher on the rubber, that locks the mechanism, period."
On Tuesday, Joe Torre, executive vice president for baseball operations, denied Maddon's protest.
Davidson stood by his decision after the game.
"I've got everything in front of me," he said. "I see Buehrle, he's on the rubber, and as I'm seeing Escobar getting ready from my judgment to get into the box, now I see Gibbons giving the thumbs up that he's coming out. So I thought, in my judgment, that it was in time to file a challenge on the play. I'm looking at Gibbons and he's coming out, and he's not a speed merchant, and I thought, it's on time. We want to get the play correct, that's what we're out here for, so that was my thinking on that."